FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720621
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC5658.220777E-714.1329731
CTTTAGA4150.002789112312.2444491
CCCCAAT4350.00396677111.6814851
CTCTGGA4750.007637760610.6977821
CTTTAAA11101.477747E-810.4637251
CTTTCAT13352.081009E-57.6126531
ATTACAG8550.0083624057.60044437
GTTTATT9700.0029317887.483721
ATTTGAT10650.00727670176.81615831
CTTTGAA12350.00420071466.46569251
CTTTATT14806.033614E-46.3763321
TAAAGAT12950.00726613266.1340282
CTTTTTT27456.61654E-55.02458861
Produced by FastQC (version 0.11.5)