FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547120
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTATCGGTCATCTCGTATGCCGT6830.12483550226641321TruSeq Adapter, Index 9 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2700.043.750118
CACGTCT2950.040.0424739
CACACGT3050.038.7296077
GCACACG4050.032.598126
AGCACAC4700.026.6114565
AGAGCAC4800.026.0570493
GAGCACA5150.024.2861844
ACGTTAC1950.008733544517.8167867
AAGAGCA8700.015.1791492
GAAGAGC8351.8189894E-1215.0063321
GTCACTA2001.8189894E-1213.20223920-24
AGTCACT2501.5279511E-1010.56179120-24
TCCACGC4650.0090176210.4601787
CAGTCAC3003.8886355E-88.33825620-24
ACTATCG3403.8829967E-87.76602325-29
ACGTCTG3054.4844273E-77.745920710-14
CTATCGG3802.7351962E-76.948546425-29
ACTCCAG4451.2491E-86.87047415-19
CGTCTGA4501.5606929E-86.79413510-14
CCAGTCA3904.2959255E-76.77037920-24
Produced by FastQC (version 0.11.5)