FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956757
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT6902.7102942E-914.6022299
CACACGT5851.3443732E-613.5324717
AGAGCAC8604.3946784E-912.5525813
ACACGTC8659.4310435E-69.9840188
AGCACAC10559.85121E-79.5502745
GAGCACA11152.0897605E-69.0363584
GCACACG9001.5819729E-48.7961056
ATGTAGC11350.00189878366.97488556
GAAGAGC22701.9234369E-55.7286831
CTTCCAT32851.0839358E-85.7180251
CTTCAAC24108.254703E-65.6956681
TGCGCCC17900.00635767965.2344942
CTTGAAT21250.00156241575.09965131
CCACCAC23108.6956104E-45.00398641
Produced by FastQC (version 0.11.5)