FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422821
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAG2450.001212930517.7603511
CTCAAAT3150.00647737113.8136071
CCCAGAA3350.00973048212.9889141
TGTTCAA4500.005250091711.2490525
TTCTATT7402.0578502E-49.7758162
CTTTCCG6400.00662328639.065181
TTTTATG8250.0059800497.8889466
AATGATT8700.00958679157.48355532
CTTTTCT10650.00733070546.8095241
CTTCTTT14304.0084377E-46.5928581
CTTTTCC12650.0054375126.30621151
Produced by FastQC (version 0.11.5)