FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782796
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACT6400.00676456239.044831
CTCGTTT13352.1793327E-57.58817241
CCCAGAT12751.0138994E-47.37774371
CTCAAAT15853.596404E-67.30434231
GTTCAAT19452.0268817E-77.06845331
CCCAAAA18456.350547E-77.05937961
CTCAAAG17852.0929374E-56.48592851
CTCCTTT32001.364242E-106.33138131
CACAAGG14150.00263780086.1364221
CAACGAA19005.213177E-56.09335851
CTCCAAT15650.00123346936.0106221
CTTAAAT18201.899046E-45.96362451
CTCTTTT40155.456968E-125.94728571
CTTTTGG22451.569466E-55.8015841
CTTTAAT26451.0796684E-65.744921
CTTTCGT16500.00232059145.70098351
CTTTGTT28406.7638575E-75.60524651
CTTCAAG23653.611074E-55.50721171
ACAATTC17150.00372022445.47844843
CTCAAAA26808.253841E-65.3998981
Produced by FastQC (version 0.11.5)