FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910009
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAA4704.7178302E-412.4535671
CTCAGCT5351.01478094E-412.3080811
ATTCGAT6705.103271E-612.0121151
CTTGGAT4750.007207308410.78216651
CCCTCGT6450.0065786129.0746921
CTCCATT8305.8188563E-48.8150241
CTTTTGT13903.775478E-67.8954711
CTCAAGA8500.0070538167.7468511
ATTCCAT12607.618862E-57.5487391
CCCATGC9700.00271631227.54275231
CTCATTT10000.00365871597.316471
CTTTGAT11850.00251001596.79166031
CTCTTCT10800.00772531426.77450941
CTTTAAT11900.00262366756.7631241
CTTTTGA15451.286062E-46.6298111
CTCTTTT20001.996592E-66.5848231
TTCCATT15451.5156719E-46.54675672
TCCATTT14756.136206E-46.3679823
CTTTTAT18753.6475074E-56.2433881
CTTTTTA18502.0359288E-45.9322731