FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009339
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT5750.002574444310.1678591
CTTAAGA7752.9684964E-49.4298681
CCCACAC10205.5066244E-58.5978211
CTCGACA10306.172913E-58.5143481
CCCAAGT8608.3900534E-48.4978471
CTCGGCT12155.417922E-68.4209121
CTCTGAT16851.3533281E-88.2406421
CTCAACT9953.9632406E-48.079361
CCCCCAT8700.0087825087.5601541
CTATAAT23451.7262209E-97.16790681
CTCTGAC11350.00161442027.08278661
CTCAAGC11450.00177132937.02092841
CTTGTAC14807.458035E-56.91311361
CCCAGCT14958.512002E-56.84375141
CCTAAAT10800.00782052156.7668041
CTCAAAC14052.9614303E-46.76198771
CTCGCGG10950.0089339986.6741081
CCCAACA13150.00103212596.66903261
CTCTTGT16554.4742254E-56.62369971
CCCGTCT12250.00360432366.56241941
Produced by FastQC (version 0.11.5)