FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530781
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCAGTGAAGATCTCGTATGCCGT9140.1721990802232936TruSeq Adapter, Index 16 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2650.069.6784067
ACACGTC3350.055.1187368
CACGTCT3350.055.1187369
AGAGCAC4000.047.9374053
GAGCACA4300.044.592934
AGCACAC4750.040.368345
GCACACG5150.038.611936
TCCGATC3050.030.2701263
CCGATCT3403.6379788E-1227.1540834
AAGAGCA8050.022.9570752
GAAGAGC8100.022.8433651
TTCCGAT7254.0745363E-1014.7059242
CTTCCGA6452.0236257E-814.3435081
GTTCAGA4756.503109E-411.985831
CTTGAAT5150.001280375311.0548921
CAGTCAC3750.010.6058120-24
AGTCACC3800.010.46625920-24
AGATCTC3400.010.44486235-39
ACTCCAG3850.010.33091815-19
CTCGTAT3600.010.25917635-39
Produced by FastQC (version 0.11.5)