FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873041
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT3052.562953E-416.7866521
TCAACGT4400.00448859611.49019053
CTTCGAG6404.235578E-511.4284121
CTCAAAT7701.9286927E-611.3987281
TTCGAGC4600.006250915610.9906172
CTCAGAT4800.007807645510.6665181
CTTTTGG11502.482666E-79.540241
CTCCTGT9001.3247257E-48.9395581
CCCATTT9954.0679366E-58.8211271
CTCTATT7700.00290464598.54904651
CTTCAAG12054.7809262E-68.49780751
CCCTGAA7850.0034582748.385691
CAACGAA7900.00366235158.3326151
CTCCAAA9702.9814252E-48.29443551
TTAAAAC9803.8150395E-48.1067822
CTTCAAT16356.982191E-88.0523121
CTTCGAT10255.3946965E-47.84936861
AATTGCC8300.00639878877.8315257
CCTCCTT11452.0898804E-47.665521
CTTCTGG11652.5524528E-47.5339231
Produced by FastQC (version 0.11.5)