FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875995
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGA4201.0508735E-515.660811
CCCATGG5008.0108026E-411.6934041
CTCCGTA4600.005723939711.1214451
CTTAGAA5550.001915951510.5345981
TACAGCG5650.002467593410.218757
CTCATAC5800.002762706710.0805221
CTTCGTT7451.992416E-49.8099031
CTCATCT8305.5209417E-59.6858021
CTTGTAG6900.0010780739.5326671
CTTCAAT15452.8558134E-109.4606831
CTCCGCT6450.00663825459.0646551
CTCCTTT17352.728484E-108.8458761
CTTGTAT10155.186074E-58.6404471
CTTTGAA14407.912786E-88.6279471
CTTCTAT15553.0249794E-88.4598591
ATATAGG7950.00437393978.1701796
CACAAAT10004.1742015E-48.0392161
CTTTTAA25301.8189894E-127.7994551
CAACAAG12305.7248948E-57.7243021
ACAAGAT11251.9899088E-47.6981253
Produced by FastQC (version 0.11.5)