FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994042
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTG2900.003900003614.9138657
CTAAAAA11552.876368E-810.0259641
CTTCGCT7552.5140797E-49.5860831
GACATCT7550.00277434828.5927565
CCCGCAC9602.9883237E-48.292961
CTTCGAA10004.636674E-47.9612421
ACAGAAC8250.0061694967.86367376
GGATTCA8300.00651297357.8163034
CAATGAA11402.2451828E-47.61841251
AAATAGC11000.00133850177.20836735
CTTGATT13301.6996481E-47.07424071
AACGAAA13100.00113786016.6110662
CTCAAAA12600.0053464696.31844571
ATCAGAA16653.901943E-46.08557941
GTTTCAT13200.0086626656.0312441
CTTTTGA14400.00319669726.0312441
CTTTGAT15800.00137723985.9548991
CTGAAAG16200.00185722155.8078641
ATTTAAT16700.00266683785.63397651
AATATTC19550.0031464245.1620025
Produced by FastQC (version 0.11.5)