FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1494359
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAGA8200.00527594948.0024221
CTTTAAT16407.779636E-88.0024221
CTCATGT8250.0055721137.9539231
CTTCAAC20257.075869E-107.92119171
CTTTCGA12205.335449E-57.7692011
CTTCATT23803.2014214E-107.3523661
CTTAAAT10000.00378833647.2910961
CTTCAAT23551.9790605E-97.1208161
CTCCTGT12908.518172E-46.7824151
CCTCAAG12150.00338829966.6009921
TTGATCT14404.778327E-46.5013853
CTTCCTT29906.7648216E-96.0962341
CTTCAGA16753.8375295E-46.094051
TGATCTT18051.8090584E-45.98465444
CTATATT15950.00141334535.9425861
CTTCAAA25805.8971636E-75.9346131
CTTTCTT36951.03682396E-105.91969971
CTTTGAT23805.716025E-65.82062341
CTTATAT15500.0066792115.6447191
CCTCAAA15700.0076944495.57281261
Produced by FastQC (version 0.11.5)