FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927301
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACT3401.6180129E-619.0592021
CCCACAC4350.004217960411.5864371
CTTAAAG8506.3682455E-711.0119841
CTCCTAT5700.002706978510.1054641
CTCAGAA6557.6720474E-49.8933271
CTCCAAT7502.4767997E-49.6001911
CTCCAAA9753.7007849E-69.6001911
CTCTGAT7904.170347E-49.1141051
CCCATCT6600.009058848.7274471
AATTAAG7550.0028331058.5727696
CTCCTTT17906.7666406E-108.4470961
CTCCGAT7900.0042206478.2026951
CTTCAAT15052.047891E-78.1330521
CTTGTAT10751.20710654E-48.0373691
ACAACTT9000.00154074957.99066733
CCCAGCT8250.0062321827.8547011
CTTCTGT13201.6503442E-47.091051
CTTTCTT24608.62201E-107.024531
CAACAAT13552.2759728E-46.9078861
TAATTAA11700.00263120126.7613345
Produced by FastQC (version 0.11.5)