FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513546
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAAAC10356.1203355E-79.8884871
TTTAACC8000.00460037788.1252856
CTCAAAT13802.9516448E-57.4163641
CTCAAAA20901.0310032E-87.3453941
CTCATTT16154.0810555E-67.24251841
CTTGAAT17702.1193846E-67.0213041
CTCAAAC11550.00193456966.96230131
CTCCAAT14454.1526233E-46.5768451
CTTAAAT13800.00177375896.35688351
CTTTGAT26852.8334398E-86.26218131
CTTCTAT21305.6790104E-66.17781641
CAACGAT14400.0028604266.09201341
CTTTTAT34002.9722287E-95.80533081
CTCCTCC15800.0080151365.55221461
CTTTATT36351.4188117E-85.4300211
CTCCTTT30001.5887617E-65.3609721
CTTCAAT27651.1894894E-55.28782371
CAAATCC23251.6887713E-45.280953
CTTCCGG33404.1207204E-75.2529941
CTTTACA19500.00255892985.2485041
Produced by FastQC (version 0.11.5)