FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870038
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT7550.023.7618777
CACGTCT7450.023.1175969
ACACGTC7850.022.853788
GCACACG12900.014.463436
AGAGCAC13900.013.4243323
AGCACAC14950.012.480155
GAGCACA13550.012.1821154
CCCAAGA9603.1327312E-59.0207621
GTCCAAC7950.004380738.1697471
CTAAAGC8900.00141127528.0630196
TCGAATT12901.2946132E-47.2332632
AAGAGCA25001.3169483E-96.89051772
GAAGAGC25802.3155735E-96.71312571
CTCATGG14555.2855705E-46.447831
AGTCACG6603.45608E-106.10028520-24
CTTTAAT18700.00177894885.40280961
GTTCTGT18450.0089193025.0848741
GTCACGT8556.3992047E-95.04534820-24
TTATTCC19950.00428930955.03585056
Produced by FastQC (version 0.11.5)