FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655414
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAG2900.003717776615.0204391
CCTCAAT4450.00469629611.4200341
CCCACAA5109.993857E-411.3880451
CTCCAAG4750.007631295410.698771
CTCAAAA9502.3863904E-69.9345721
CATGGTT5900.00336153629.8439041
CTTTAAT10609.2880146E-79.5885191
CTTAAAA8558.207475E-59.3401961
CTCTATT6650.0090000878.7336881
CTCATTT7850.00369654488.3234281
CTTCAAA12901.31569395E-57.87893871
TTCAATT10155.4574007E-47.84021952
CTCTTTC11251.8558824E-47.74387071
CTTCTGT9550.00251365347.6019681
CTTTCTT18659.1014954E-87.3961231
CATCAAT12003.9052236E-47.2598791
CTTTGAA11250.00157283797.09854841
CTTTTAT14757.750692E-56.89073231
CTTTTCA16203.628173E-56.722111
TATTGAT12959.7451353E-46.7011197
Produced by FastQC (version 0.11.5)