FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571198
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2152.5424015E-829.3030039
CACACGT2253.954119E-828.0006477
ACACGTC3052.9460352E-822.9513518
AGAGCAC3301.5912701E-619.089683
GAGCACA3802.9997318E-718.4214784
GCACACG4157.560957E-716.8663836
AGCACAC3806.1146693E-616.5778795
GAAGAGC6951.2243119E-511.101751
AAGAGCA7703.874296E-510.0019832
CTTGATT6400.0088465078.7517352
TGTGCCG7600.00383315218.2896657
TTGGCTA2901.00484336E-46.757830630-34
Produced by FastQC (version 0.11.5)