FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1792411
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT8500.015.8453797
CACGTCT8500.015.8453799
ACACGTC9050.014.88240058
GCACACG12850.012.5776566
AGCACAC12650.012.2441575
AGAGCAC14856.366463E-119.5250943
GAGCACA17351.8189894E-129.315444
GGTAAAT13504.6375647E-46.5175951
AAGAGCA26451.1042175E-75.8581892
AAAAAAA33150.05.40392260-64
GTCAGAT16500.00511273285.3325771
GAAGAGC29559.287469E-75.2680281
GGTCGAT19400.00110769355.23319961
AGTCACT7904.127287E-95.13346520-24
Produced by FastQC (version 0.11.5)