FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691926
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAAT3456.2499446E-414.9301021
CTCAGAT3400.00975578612.9854251
CCCCCAT4500.004616490611.4464111
CCCAGTT4750.00690474810.8439691
CTTGCTC4900.00869686110.5120111
CCCATAA5750.002428560810.2377841
CTCCCAT5900.00300679619.9775021
CTTCGAT6350.0055169879.2704351
CTTAAAA9201.5740815E-48.7980971
CTCAAAA9351.875892E-48.656951
TTAATAT9352.2944962E-48.4974243
ACAAGAT7750.00344873578.387783
CTTCTGT8400.0060232487.8840081
CAACAAA9800.00283767457.50857931
ATATTCC12355.757467E-47.0181156
CTCTTTT12900.00574175046.27461051
CTTCTTT18350.00665110775.2130411
Produced by FastQC (version 0.11.5)