FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916898
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT5002.7237547E-816.1722139
CACACGT4351.841403E-615.4897797
ACACGTC4957.0293117E-613.6129738
GCACACG5903.0931224E-612.5624746
AGCACAC6257.670887E-510.7808875
AGAGCAC7152.6178128E-510.3662383
GTCCTGT5300.002444638610.2303441
GAGCACA6601.3323395E-410.2091734
CTTAAAT5500.0033231929.8583311
GACGACC5650.0043564369.5406076
CTTATGC5850.0055294399.2685171
TGTTATT9550.0051750617.06055972
ATTTACC11150.00314514786.64740756
CTGAAAA19351.7406243E-45.60421941
CCATTTT14650.0085489775.5192254
AGATGAT14800.0089663765.49535371
GATTTTC17050.0078969435.13751945
CTTTTCT18600.00364442525.1014221
Produced by FastQC (version 0.11.5)