FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3515341
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT9300.0020048377.7812091
CCCAAAT22204.0006853E-86.84536171
CTCTCGA14253.9710134E-46.6017421
CTTTAAT37600.06.5436661
CCCACTT22905.06001E-76.32010941
CTTCAAA54250.06.26943161
CTTAAAT26051.2643795E-76.1114611
CTCCGTT32151.0113581E-96.07733061
CTCCTAT21558.111418E-66.04442931
CTCAGAA19608.183696E-55.90736681
CCCCGTT19658.4908635E-55.89233541
CTCGAAG28401.0980693E-75.8605661
CTCAAAA39902.7284841E-115.803731
CTTCAAT50200.05.62200161
CTTATTA32404.6504283E-85.58373831
CATCAAT58400.05.576091
CTCCCGT24901.3915658E-55.52184631
CTTAATT23703.7351663E-55.4960951
CTCAGAT15800.00897271655.49609471
CTCCCAT26356.136692E-65.49261861
Produced by FastQC (version 0.11.5)