FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857331
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC6556.7181577E-410.0371641
CCCTGTT7000.00123506559.3919181
CTTTAAT11301.9944711E-58.4037811
CTTCTCT10507.753806E-58.34837151
CTTCAAT12859.909987E-57.3900951
CTATTAT11953.4711967E-47.33538871
CTTTATT16154.117088E-67.23697761
CAAAAGA16052.9290184E-56.8269391
CTCTTCT10900.0085739086.70167451
CTTTTAC13200.00122904136.56595666
AAGAATC12150.00373607696.5389364
CTTTGAT14700.00362712735.9631231
ATTTGAT13500.0099248565.952081
CTTTTAT18602.2387013E-45.8909881
CTTTCTT22156.838891E-55.60641151
AAAGAAT23652.173568E-45.1916863