FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017249
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTT4451.8629147E-514.7420261
CACCTCG4953.1168074E-614.7254811
CCCCAAT3950.001867257112.9174151
CTCCGTT7100.00143420799.239721
CCCTGAT8004.1835854E-49.1113911
CTCCAAT8004.1835854E-49.1113911
CTTACTG6600.0081932698.8352891
CCTCATT9352.0809508E-48.5754271
CCTCTAT8150.0050030978.0493271
ACGATTT11101.7318214E-47.7914033
CTTTAAG11351.9657634E-47.7065511
CTTTGAT19904.2127795E-97.6920291
CCCATTT10809.784949E-47.42409661
CTTCCAC13853.2141892E-57.3680561
CCTATTT10100.00418254037.21694371
CTTCCAT18304.895519E-77.1696191
CCTTGAT13251.4868006E-47.15158271
CTTTTAT25902.746674E-107.0358231
CTTTCAT19702.2218592E-77.03010851
ATTCAAG12808.8876253E-46.7566076
Produced by FastQC (version 0.11.5)