FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594544
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGA9753.3443157E-69.6780281
CCCTATT7850.00370876548.3218721
CACTATT9853.8243065E-48.1059641
CTCAAAC12609.666139E-68.0650241
CCCAAAT11452.2863715E-47.6071841
CGCCTAT8600.0084160967.5961271
CTTCGCT13552.5462694E-57.49957941
GATCCAG10100.00451421977.1611725
TTCGCTT14204.945761E-57.1322562
CTCACCG14556.5182336E-56.9841441
CTTAAAG14808.150961E-56.86616951
CAACAGT12858.57713E-46.77838561
CTTTGAT23751.9157596E-86.7236831
CCCAGCT13050.00102219596.6745031
TTCGATT12050.00337373056.60378462
CCTATAT13600.00163061896.4045781
CTTTGAA26101.8339051E-86.39639851
CTCCAAT12500.0047783876.38749931
CTTCCGA14806.05592E-46.37572861
GTTATAT17107.7272125E-56.3671241
Produced by FastQC (version 0.11.5)