FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350199
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAT13102.2666192E-57.56637141
CCCTCGT15905.224061E-67.12449031
CCCAAAT19201.7606744E-66.6374641
CTCCAAT23401.6448575E-76.35381171
CTTCTAT36701.891749E-105.78743461
CCCAGCT29405.648144E-85.7795611
CTAAATC15100.00710689845.61361032
AAACTAC16700.003567155.49835065
CAACAAT45451.0913936E-115.45211651
CTCAAGA20952.9133918E-45.40713071
CTTCAGA31802.9066723E-75.34336761
CCCTTGT22701.593222E-45.3021741
CCCAGAT28104.9126593E-65.29107481
CCTCGGT22951.8772266E-45.2444161
CTTGTTT45202.6921043E-105.16899871
AGCACAC19250.00335198135.1369215
CTCAAAT26254.8164384E-55.12454371
TCAGATT43603.1268428E-95.0224222
Produced by FastQC (version 0.11.5)