FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911416
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCAT16007.2759576E-129.9385341
AGATTCT12358.27789E-68.1577524
CAAGAAT13954.7230224E-67.772071
CTATCAA9800.00338252427.37553551
CTTCAAT13602.270736E-46.90914151
CTTTCAT17903.0131068E-66.8646051
CAGATTC11700.00261306166.7657543
GAAATCA20007.315198E-45.39722735
Produced by FastQC (version 0.11.5)