FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326498
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAT14155.9117156E-109.8051771
CTTAATT7602.4577283E-49.6082581
GTCCCAT6700.0091339248.7191351
CTCTACT8457.096345E-48.64174651
CTCAGCG9703.037431E-48.2809311
CCCCATT8450.00681234437.77757171
CTCAACT9450.00214449397.7272761
CTCCAAC10708.698229E-47.50701241
CTTTCGT10950.00111223757.3356191
CTCAAAT10050.0039174787.2659461
CTCCATG14857.869905E-56.88429981
CTCATTT12807.659538E-46.84588341
CCTCAAT10700.00719995756.8245571
CCTCTAT11850.00256402886.77848341
CTCCTTT20654.891299E-76.71880051
CTTTGAT19601.481867E-66.7061721
CCTTGAT27358.9858077E-106.6748411
CTCTGAT10950.0089987256.66874461
CTCAGCC11050.0098224216.60839461
CTTTCAA20002.0658154E-66.57204771
Produced by FastQC (version 0.11.5)