FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281523
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4703.4078763E-513.8280828
CACACGT4450.004890883311.3593987
CCCTCGT5300.00122870111.1117421
CACGTCT4700.007358392210.7526579
CTCCAGG7151.2239988E-410.2958451
CCCCAAT5750.00242247910.2421271
CCCACTT6000.00344701279.8153721
CCCACGA6100.00395170239.6544651
CTCAGAT6350.00550322759.2743681
GAGCACA7200.0017745829.0266654
CCCACAA9151.4794714E-48.8499261
CAACGAG8809.799197E-48.3653741
CTCCGAA10759.3339535E-58.2175221
CCTGTGT9450.00198059027.7899781
CCCAAAG9450.00198059027.7899781
CACCGAT16505.0609997E-67.13845251
CTTTGAA19601.7129969E-77.13617661
CAACAAC17901.6910055E-56.58013771
CTTGTTT16252.2911513E-46.3422411
CTCATTT13150.006988856.15793371
Produced by FastQC (version 0.11.5)