FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975248
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3707.701401E-515.4205488
CACACGT3707.701401E-515.4205487
CACGTCT3951.3531449E-414.4334559
CTCACAC3050.005722143714.0771261
CTTTAAG8154.9559203E-610.5362531
GAGCACA6305.855589E-410.1885784
AGCACAC5850.0036318569.7531685
AGAGCAC6950.00143984579.2356893
CCCATAT7854.1632453E-49.1157611
CAATCCT9603.376745E-48.1994361
CTTCGGA10851.4442013E-47.91432861
AAAGATC10055.7252415E-47.8061744
CTTCTGT15252.3776156E-67.50779961
CTTTTAA10559.2795654E-47.46109871
CTTTAAT9900.00411945677.22815371
CTTTCCT13502.346358E-46.89083961
CATACAT10450.0069639136.8477241
CTTCGTC12150.0041179636.47856661
AAGAGCA13600.0019809386.29423672
CTCCTTC13900.0024434686.1777311