FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436463
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1652.5423778E-630.5022187
ACACGTC1907.621202E-626.488778
CACGTCT1907.621202E-626.488779
AGCACAC2351.4275356E-624.4759435
GCACACG2151.9849469E-523.411366
AGAGCAC2659.916168E-519.0006563
GAGCACA2751.3173444E-418.3097234
TCTGCCA8000.00477810588.0885357
CTTTTGC9650.0029020127.49005461
TCTCGTA2350.00271113036.743091635-39
Produced by FastQC (version 0.11.5)