FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694891
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAGA4654.6814134E-412.466221
CTTGTAT11051.6700133E-58.5246941
CTTAATA8950.00135521818.0960791
CTAAAAG8100.00499676268.0511011
CTTCTAT12155.4783588E-57.75291161
ATTCAAG10150.00465345347.13890651
CTAAAAT12506.3871354E-46.9561511
CTATAAA12657.317849E-46.8736671
TTCGCTT11650.00238497676.82555532
CTTCCAC18705.7557972E-66.5872641
CTTGAGC14900.0046206795.83569671
CTTTGCT14900.0046206795.83569671
AACGACC15100.00550537465.74436
CCTCGAT27954.835874E-75.70346261
CTCCATT15350.00642593255.6646181
TTCGATT16750.00280618065.6104772
CTTCGAT20952.0902525E-45.5339311
CTTAAAA18350.00133108935.52827641
CTTCAAT17150.00360830695.4925881
CTTTTGT18650.00163372285.4393491
Produced by FastQC (version 0.11.5)