FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028091
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCAAT4800.00786409510.65646651
CCTCGAA8305.5325214E-59.6843611
CTTCAAT15653.601599E-98.8714721
CCCGAAT8759.961332E-48.351191
AGAATGC9703.42184E-48.1894565
CCCGTTT9853.5551185E-48.1604261
CTTCAAG13552.6743091E-68.08925151
TTATGGT8500.0079466757.64455279
CTCAAAA14456.669685E-67.5854231
CAACGAT9950.0035279087.34401131
CATGAAC10850.0081784216.734831
TCGAAAT13150.00117974246.5900633
GTTAAAA12350.0039245596.50851771
ATTTAAT15851.8239797E-46.4543891
ATTCCGC13500.0015876226.41921048
ACAATAT16907.03697E-56.40971373
CTTATAT12900.00618047836.2310231
CAACAAG14150.00235989066.19699571
CTTAAAA14300.00265606386.13199231
CTTATTT14600.00335030076.0059931