FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966625
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTAG1050.00860073127.0057649
CACGTCT3252.67174E-517.4498799
ACACGTC3606.4174834E-515.7533628
CACACGT3600.001144477913.7841917
GCACACG6053.2831194E-511.7173776
CTCGCGG6305.9019646E-410.1798191
CTCAATC6700.00103836439.5720691
AGCACAC7050.00173290389.0498045
GTTTTGT11955.4777513E-57.75199651
GAAAGGC11800.00334577846.6084027
GAACTGC12950.0088072826.0215565
GACCCGA3600.0082430795.9382281
AGAAAGG14800.00546114945.7478486
GTCTTTT14900.00555555855.73895841
GATAATC15450.0087585135.50716922
Produced by FastQC (version 0.11.5)