FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218316
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC3200.006874167413.6930571
CTTACCT8303.9010774E-711.4383761
CTTCGAG7802.2967542E-611.2353291
CCCAACA7452.008824E-49.8026361
CCTTTAG6050.00434015139.54545
CTCCCAC7953.856672E-49.1861171
CTCAAAT12004.712274E-79.1287041
CTATCGT6650.0085832828.7855211
CCTAAAG6650.0085832828.7855211
CTTGGAT8608.443339E-48.4918181
CTTTGAT20851.6370905E-118.4062891
CTTAAAT11853.634395E-58.0116891
CTTATGT8350.0061168737.87145761
CTCCGTT10456.7500764E-47.68733071
CTTCCGT15301.8417213E-67.63708641
GGTTGTT11903.3209912E-47.3643331
CTTTCCT18154.1403655E-77.24260851
CTTATTT22254.5929482E-97.2209081
CTTGAAC12204.4199673E-47.18324331
CTTCGGG13351.5942437E-47.11150031
Produced by FastQC (version 0.11.5)