FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2512304
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAT11653.4417099E-79.3303041
CTCGCAG14600.00368340455.95605751
CTCTTGT14700.0039745115.915541
CTCTGAA14950.004794425.8166181
GTTGCAT17607.8331935E-45.76429031
CCCAGTT19354.2817596E-45.61747031
CTCTCTT18550.00152615965.46908431
GTGCAAT19956.4613123E-45.44852351
GGTAAAT26854.761637E-55.1279031
CTTAAAG22857.155526E-45.07416151
Produced by FastQC (version 0.11.5)