FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432582
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGTT4051.8922947E-819.6750721
CTCAACA3750.001320798613.522141
CTCCGAT3250.00802512713.3735451
CCACAAT3300.00887691613.1709161
CAAATCT4100.002645610612.3292033
CTCCCGG6002.4096973E-512.073341
CCAGTTT5700.00263675710.1352832
CTCAAAA6000.00392862369.6586721
CTTCAAT7703.0267707E-49.4077971
CTTATAT6450.0071262298.9848111
CTTTCAT8256.034087E-48.780611
Produced by FastQC (version 0.11.5)