FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591127
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTGGCACTAATCTCGTATGCCGT6490.10979028195294752TruSeq Adapter, Index 4 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3150.040.8894587
CACGTCT3350.038.448299
ACACGTC3350.036.3122758
GCACACG4900.029.2067556
AGCACAC5000.027.1914865
GAGCACA5450.022.320394
AGAGCAC5750.021.1558483
GACGTAG2608.92434E-519.2652235
AAGAGCA9750.014.6981582
GAAGAGC9900.014.4987931
GCACTAA2053.092282E-1112.56921830-34
AGTCACT2651.8189894E-1211.34257320-24
ACTAATC1902.0854714E-811.3012730-34
CACTAAT1953.058267E-811.01149430-34
CTAATCT2109.0743924E-810.22495830-34
GGCACTA2252.280649E-810.17951530-34
CTCGAAA6300.00634638739.1135271
ATTGAAT6300.00634638739.1135271
CAAAGCT6500.0082014588.8331111
GTCACTG3251.1095835E-98.80815120-24
Produced by FastQC (version 0.11.5)