FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961453
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGTT6755.758091E-611.8829951
CCCACGA4600.005822016411.0962741
CTCATTT9451.8134051E-710.8027231
CCCAAAA9752.788911E-710.470331
CTCAAAT8759.234673E-610.0002341
CTCAACG5900.003243849.8872371
ATCGTCG7853.912475E-49.1725156
CTTCAGA9753.2579573E-48.2266881
CTCCAAT8000.00421796048.2033171
CAAAGTT10155.0197495E-47.9024841
AGATTCC9250.00199394287.78424176
CTTCCAT16406.5930726E-77.5586121
CTTTCCT12455.6695705E-47.02827741
CTTTCAT19009.0651884E-76.9080571
TCATCGA11600.00237340186.82798864
CTTCCGT11850.0026010286.7687941
CCATGAT11950.0028411816.71215151
CTCTTTT20002.1110554E-66.5626541
CTCAAAA13400.00130825296.53000351
TCAAATT15802.1190329E-46.38012272
Produced by FastQC (version 0.11.5)