FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468059
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT1950.046.011729
ACACGTC2500.038.649848
CACACGT2550.037.8920027
GAGCACA3101.8189894E-1228.9397624
GCACACG3350.028.8400866
AGCACAC3201.8189894E-1228.0353955
AGAGCAC3355.456968E-1226.7772183
GAAGAGC6551.3278623E-1015.8427931
AAGAGCA6654.508911E-813.49362
AGTCACT2203.0396404E-79.41048220-24
ACTCCAG2707.086783E-88.68943115-19
CTCGTAT2759.522955E-88.53180635-39
CTTTTCT10701.9837475E-58.405071
TCTCGTA3004.3697582E-88.2808735-39
TTGGATC2253.8573766E-57.974512630-34
GTTGGAT3550.0016206947.79495241
CAGTCAC2704.732232E-57.15661420-24
GTCACTT3309.4831375E-57.03461145
ACGTCTG2951.9982417E-57.01798710-14
CTGAACT3609.256146E-76.9010210-14
Produced by FastQC (version 0.11.5)