FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622895
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT12000.014.8771559
CACACGT12150.014.1054777
ACACGTC12550.013.0871548
AGCACAC16450.011.7206235
GCACACG17550.011.3928866
AGAGCAC15550.011.0234243
GAGCACA15850.010.3621864
CTCGAAT12357.543455E-57.55634831
GTTAAAT12950.00106301876.65192031
CTCACGA12350.0047320766.3938331
GAAGAGC30151.1490556E-85.95234731
TTATAGG15950.00181455995.8201686
GTTTAAA15400.00738994875.59366041
AAGAGCA32406.125629E-85.5132
CCCAAAT19756.4058125E-45.4520491
CTTAAAA22155.272508E-45.18539521
Produced by FastQC (version 0.11.5)