FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647183
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT4251.2498511E-515.3755361
CTCACAG3000.00465395214.5213411
CAACAGG4652.923267E-514.052911
CATCTTA3250.00790969413.4043141
CCTACAT3300.00874934113.2012191
CTCCTCA3300.00874934113.2012191
CTTCACT4050.002318796212.5493061
CTACGAG4400.00431291611.5510661
CCCAGAT6053.4140123E-410.8009961
CCCTTGT4750.007624936310.6999361
CTTCGAC9552.5482805E-69.8836351
CTTCAAT7750.00328896668.4317461
CCTCCTT9050.00147934868.022841
CTTTCAT9200.00173962367.89203261
TTCATAT11050.00135797687.19763333
CTCCTCC10250.0050090977.0835811
CTCCTTT11300.00164641277.0679091
CTTTCCA10750.0079464456.7541121
CTTCTTC18404.310121E-66.70822761
CTTCTTT14303.9624132E-46.60060931
Produced by FastQC (version 0.11.5)