FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812800
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGA2050.00959922317.5280083
CCCAGTC4001.261039E-414.5529761
TGTACGC4250.003595104711.836567
CTCATGA4350.003897622511.709291
CTCCCTT5900.00329996839.8664241
CTTATAA8751.0310331E-49.1475851
CACCGTT7700.00304322328.5049861
AACGATT7950.0045433228.1356413
CCACCAC9950.00414869847.22259578
TTTCTTA13050.00114260926.608267
CTTTCTT19651.1593618E-56.295181
ATTCAAT13100.00756996.1100281
TTCAACA13050.0082768386.0575723
AAAATAG16854.9772346E-45.9709655
AATATTG14600.00403194465.9066985
Produced by FastQC (version 0.11.5)