FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056722
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGA5651.876775E-411.5224321
CCAAGTT5950.0037177859.725751
GTTCGTT6700.0098658868.6370461
CTCCAAT7750.0034058698.4002251
TCCGATT9653.3226574E-48.21192
GTTTAAT9350.00211894287.7363921
CTTGAAT11251.9390463E-47.7157621
CTTCACT8450.0074127.7043481
GATCCGC8550.0085761987.57964945
TCAGGTA8550.0085798247.57929139
CAACGAA8700.009617317.4829591
CCTCGAT9750.00319477127.41900161
CTTTCGG12255.165661E-47.08590361
CATCAGA11700.0057778446.9809074145
CTTTAAT14758.147689E-56.86572071
CCAAAAC10550.0068800086.8564231
CTCCAAA10800.0086270636.69770961
GGGGGGG2200.0097112716.617012110-114
CAAAGAG14400.00321686696.0279391
AGATTTA15750.0014097795.94340754
Produced by FastQC (version 0.11.5)