FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153222
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6050.027.1378927
CACGTCT6850.022.9273879
ACACGTC7150.020.9669728
AGCACAC9800.018.2103185
GAGCACA9450.017.3747464
GCACACG10350.015.8632116
AGAGCAC9650.015.5351143
CTAAGGT3100.006234392513.8964811
CTCTCGT6709.6935057E-49.6445721
AAGAGCA17657.2759576E-129.3066622
GAAGAGC17806.91216E-118.873971
CAAATTT9905.102912E-58.6530363
CTTCGAT11450.00213350686.89767071
CTTCAAA17951.7458301E-45.99987361
CTTCAAC17106.09014E-45.8782381
CGCTCTT15550.008730375.5090074
CTCCAGC17500.00509627545.33360151
CTGGAAA17750.00601942055.25848051
AAAAAAA27100.05.111153660-64
CAGTCAC7351.6494232E-75.06380920-24
Produced by FastQC (version 0.11.5)