FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018602
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAG8558.781845E-59.2852441
CTCACAG15905.489528E-88.1703791
CTCAGAT18005.4546035E-87.61812161
CCGCGTA9650.00297756757.47360378
CTCAAGG17502.753095E-77.42337231
CTCCCGT15803.5700086E-67.3085241
CTTCGAT26306.548362E-117.13484241
GGGGGGG2505.402432E-47.0598025135-139
CCCAGTT23903.3433025E-96.9453911
CTCAGGA19853.0283263E-76.908121
CCCGCGT10700.0080707826.74501661
CCCACAG17151.2074059E-56.73321721
CTCAAAT25752.220986E-96.72668031
CTCCCGA19401.5222377E-66.69634061
CCCAAAT21651.9243816E-76.667131
CTCAGTT14354.4663655E-46.5382011
CTCCGAG12150.00377302176.53406141
CTTTCGT27651.4988473E-96.52546831
CCCTCCT17752.0040723E-56.5056161
CCTACTT13400.00147270026.46313521
Produced by FastQC (version 0.11.5)