FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974010
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGGGT4750.00794080110.6424648
GTCAGTT6000.00402756739.6318632
CTCCAGG7350.00207076228.8742991
AGTAATA7650.00307339668.4960845
CATCGAT8700.0094536897.4972531
CTTAAAA11652.838489E-47.4650751
CATAAAG10150.00464074037.14024071
CCCAAAA10300.0053527.03625631
CTTGAAG13451.9183905E-47.0048681
CTTTCAT13451.9183905E-47.0048681
CCTCCGG13752.5144295E-46.85203461
CGTTGAT10750.0080973776.74171541
CTCAAAG12000.00316557926.6433991
CTTCAAC13300.00128821736.5389571
CTCAAAA12650.00549287446.30203871
CCTTGAT12750.00596164076.2526111
CTTTTGT15258.849398E-46.17806431
AAAAGGT13100.00816443756.0658683
CGATGAT16904.643361E-46.0037171
CTCGCCG25401.883419E-55.42124181
Produced by FastQC (version 0.11.5)