FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322956
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4854.967458E-513.2847687
CACGTCT4756.1969983E-412.0572879
ACACGTC5150.001220437911.1207988
GCACACG7452.454795E-49.6094156
AGCACAC8751.2325762E-48.9999035
CTGCAGT10350.0060046336.95371531
CTCAAAG15651.8856944E-46.43829631
CACAAAT15752.0481463E-46.3974181
CCCGGAA17100.00377424555.4714761
GTTTCAT19906.838675E-45.42494631
CAGATTC18800.00209859395.33198643