FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993184
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGG9854.3694934E-58.7691011
CTCAGAT10256.965102E-58.4268921
CGACTAT11000.00136086147.1979711
CTTTGAT32705.456968E-126.60364251
CACGATC13150.00128204336.54261352
CTGTGAT19008.178586E-66.440291
CTCATAG13900.00229121636.21407561
CCCGCCT15750.00141815035.9411821
CTCAAAA26251.0343301E-65.75837661
CTCAAAC15550.0079792215.5547041
CTTCGAT18700.00183807175.3888551
CTTTTAT36502.240813E-85.32452631
CCTCCAT21854.1824178E-45.27082541
CAAACAT22004.607673E-45.23488761
CCCGAAA22405.942431E-45.1414081
CTCCGTT18250.0080966215.12732171