FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519151
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACGGAAGGATATCTCGTATGCCGT6040.11634379978079595TruSeq Adapter, Index 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2250.050.102238
CACACGT2600.046.067567
CACGTCT3200.037.4298869
GCACACG3700.032.3686756
GAGCACA4100.030.9290374
AGAGCAC3900.030.7057883
CCCCCCC453.7691422E-430.592411140-144
AGCACAC4550.026.3217835
GAAGAGC7100.017.8949051
AAGAGCA7650.016.5795422
AAGCAAC4750.009531666510.3820286
ATTGCAG8409.372377E-59.2264158
AGTCACG2705.0818926E-88.870048520-24
TTCATTG7750.00430748848.1828062
CTCGTAT3105.3831172E-88.17965435-39
CAGTCAC3301.7958882E-88.11111420-24
ACTCCAG3608.365532E-87.43518815-19
TCTCGTA3259.909363E-77.368679535-39
TCGTATG4551.4830221E-86.81151640-44
ACGTCTG3902.4591554E-66.503529510-14
Produced by FastQC (version 0.11.5)