FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753511
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT5053.764837E-614.458631
CTCAAAT6452.3042776E-713.5843881
CACAACG4350.003798076411.7497151
CTTCGAT7702.4426656E-510.4308691
TTGGAGT5750.0028539610.0407759
CCTACAA6556.743366E-410.0327441
CCTCAAT6758.8873645E-49.7354771
CTCTTAT6150.00451852479.4980271
CTTTGAG7300.00181749239.0019831
AAAGATC6600.0088810558.7482254
CTTTAGA6700.0091330838.7183381
CTTACAA7750.00312713028.4792871
CTTCTGG8650.0083965177.59704881
CTTCTGA10050.00391619337.2652821
CTCCTTT15351.5778047E-57.13512231
AGATTTA10850.00921045156.65187644
CTTCTTC24351.2850342E-65.99721431
CTTCTTT24451.3824992E-65.97268531
CTTTTAT16350.00186404265.805561
CTTTTTC35508.336428E-95.55333571
Produced by FastQC (version 0.11.5)